Botany

Technique Involved in Plant Tissue Culture – PTC

1. Sterilization: Sterilization is the technique employed to get rid of microbes such as bacteria and fungi in the culture medium, vessels and explants. i. Maintenance of Aseptic Environment: During in vitro tissue culture maintenance of aseptic environmental condition should be followed, i.e., sterilization of glassware, forceps, scalpels, and all accessories in wet steam sterilization […]