The green fluorescent protein (GFP) is a protein containing 238 amino acid residues of 26.9 kDa that exhibits bright green fluorescence when exposed to blue to ultraviolet range (395 nm). GFP refers to the protein first isolated from the jellyfish Aequorea victoria. GFP is an excellent tool in biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, enzymes or substrates other than molecular oxygen. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors.
Agro forestry Agroforestry is an integration of trees, crops and livestock on the same plot of land. The main objective is on the interaction among them . Example: intercropping of two or more crops between different species of trees and shrubs, which results in higher yielding and reducing the operation costs. This intentional combination of […]
The protein called starch branching enzyme (SBEI) is encoded by the wild-type allele of the gene (RR) which is dominant. When the seed matures, this enzyme SBEI catalyzes the formation of highly branched starch molecules. Normal gene (R) has become interrupted by the insertion of extra piece of DNA (0.8 kb) into the gene, resulting […]
Apart from monohybrid, dihybrid and trihybrid crosses, there are exceptions to Mendelian principles, i.e. the occurrence of different phenotypic ratios. The more complex patterns of inheritance are the extensions of Mendelian Genetics. There are examples where phenotype of the organism is the result of the interactions among genes. Gene interaction – A single phenotype is […]