Botany UG degree

Insertional Inactivation – BlueWhite Colony Selection Method – DNA screening methods

It is a powerful method used for screening of recombinant plasmid. In this method, a reporter gene lacZ is inserted in the vector. The lacZ encodes the enzyme β-galactosidase and contains several recognition sites for restriction enzyme. β-galactosidase breaks a synthetic substrate called X-gal (5-bromo-4-chloro-indolyl-βD-galacto-pyranoside) into an insoluble blue coloured product. If a foreign gene […]