Botany

Technique Involved in Plant Tissue Culture – PTC

1. Sterilization: Sterilization is the technique employed to get rid of microbes such as bacteria and fungi in the culture medium, vessels and explants. i. Maintenance of Aseptic Environment: During in vitro tissue culture maintenance of aseptic environmental condition should be followed, i.e., sterilization of glassware, forceps, scalpels, and all accessories in wet steam sterilization […]

Botany

Plant Tissue Culture

Plant Tissue Culture (PTC) Plant tissue culture is used to describe the in vitro and aseptic growth of any plant part on a tissue culture medium. This technology is based on three fundamental principles: •The plant part or explant must be selected and isolated from the rest of plant body. •The explant must be maintained […]