Genetic engineering or recombinant DNA technology or gene cloning is a collective term that includes different experimental protocols resulting in the modification and transfer of DNA from one organism to another. The definition for conventional recombination was already given in Unit II. Conventional recombination involves exchange or recombination of genes between homologous chromosomes during meiosis. Recombination carried out artificially using modern technology is called recombinant DNA technology (r-DNA technology). It is also known as gene manipulation technique. This technique involves the transfer of DNA coding for a specific gene from one organism into another organism using specific agents like vectors or using instruments like electroporation, gene gun, liposome mediated, chemical mediated transfers and microinjection.
Terminology related to Mendelism Mendel noticed two different expressions of a trait – Example: Tall and dwarf. Traits are expressed in different ways due to the fact that a gene can exist in alternate forms (versions) for the same trait is called alleles.If an individual has two identical alleles of a gene, it is called […]
Mutagenic agents The factors which cause genetic mutation are called mutagenic agents or mutagens. Mutagens are of two types, physical mutagen and chemical mutagen. Muller (1927) was the f irst to find out physical mutagen in Drosophila. Physical mutagens: Scientists are using temperature and radiations such as X rays, gamma rays, alfa rays, beta rays, […]
Single Cell Protein (SCP) Single cell proteins are dried cells of microorganism that are used as protein supplement in human foods or animal feeds. Single Cell Protein (SCP) offers an unconventional but plausible solution to protein deficiency faced by the entire humanity. Although single cell protein has high nutritive value due to their higher protein, […]